realplex abi 7000 (Eppendorf AG)
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realplex abi 7000
Realplex Abi 7000, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 204617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/realplex+abi+7000/Eppendorf/pmc02920330-153-18-20
Average 99 stars, based on 204617 article reviews
Realplex Abi 7000, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 204617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/realplex+abi+7000/Eppendorf/pmc02920330-153-18-20
Average 99 stars, based on 204617 article reviews
realplex abi 7000 - by Bioz Stars,
2026-10
99/100 stars
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Quantitative RT-PCR:Article Title: Human pluripotent stem cell-derived hepatocytes support complete replication of hepatitis C virus. Article Snippet: Background & Aims: Worldwide, about 180 million people are Conclusions: Hepatocytes derived from hESC support the comchronically infected with the hepatitis C virus (HCV).. Current in vitro culture systems for HCV depend chiefly on human hepatoma cell lines.. Although primary human hepatocytes support HCV infection in vitro, and immunodeficient mice repopulated with human hepatocytes support HCV infection in vivo, these models are limited because of shortage of human livers to isolate hepatocytes. SYBR Green Assay:Article Title: Human pluripotent stem cell-derived hepatocytes support complete replication of hepatitis C virus. Article Snippet: Background & Aims: Worldwide, about 180 million people are Conclusions: Hepatocytes derived from hESC support the comchronically infected with the hepatitis C virus (HCV).. Current in vitro culture systems for HCV depend chiefly on human hepatoma cell lines.. Although primary human hepatocytes support HCV infection in vitro, and immunodeficient mice repopulated with human hepatocytes support HCV infection in vivo, these models are limited because of shortage of human livers to isolate hepatocytes. Article Title: Hmga2 translocation induced in skin tumorigenesis Article Snippet: RNA was isolated using the RNeasy Micro-kit (Qiagen), and DNase treatment with the Turbo DNAse kit (Ambion). cDNA synthesis was performed from 1 μg of RNA with Superscript III First-Strand synthesis system (Invitrogen). .. Quantitative Real time PCR (qRT-PCR) was performed using the Platinum SYBR green qPCR Supermix-UDG (Invitrogen) and the Article Title: Human Embryonic and Rat Adult Stem Cells with Primitive Endoderm-Like Phenotype Can Be Fated to Definitive Endoderm, and Finally Hepatocyte-Like Cells Article Snippet: DNAse treatment was performed using Turbo DNAse kit (Ambion 1907). cDNA synthesis was performed from 1 μg of RNA with Superscript III First-Strand synthesis system (Invitrogen 18080-051). .. Real time PCR was performed with SYBR Green Platinum SYBR green qPCR Supermix-UDG (Invitrogen 11733-046) in an Article Title: Lack of myostatin reduces MyoD induced myogenic potential of primary muscle fibroblasts. Article Snippet: Abbreviations used: αSMA, alpha smooth muscle actin; bHLH, basic helix loop helix; CAR, Coxackie and adenovirus receptor; CDK, cyclin dependent kinase; DMEM, dulbecco’s modified eagle’s medium; PBS, phosphate buffered saline; EDTA, ethylenediaminetetraacetic acid; Mstn, Myostatin; Mstn, myostatin null; MPP1, muscle preplate1; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; TGF-β, transforming growth factor beta; WT, wild-type; Article Title: NKX2-1 Activation by SMAD2 Signaling After Definitive Endoderm Differentiation in Human Embryonic Stem Cell Article Snippet: RNA was isolated using the RNeasy Mini-kit/Micro-kit (Qiagen), and DNAse treatment with the Turbo DNAse kit (Ambion). cDNA synthesis was performed from 1 μg of RNA with Superscript III First-Strand synthesis system (Invitrogen). .. Real time PCR was performed using the Platinum SYBR green qPCR Supermix-UDG (Invitrogen) and the Article Title: Non insulin producing cell line, MIA PaCa-2 is rendered insulin producing in vitro via mesenchymal epithelial transition. Article Snippet: We used non-insulin producing pancreatic carcinoma cell line, MIA PaCa-2 and have modulated its culture conditions by using 1% matrigel as extracellular matrix, N2, B27 growth supplements and serum free conditions.. Expression of markers was analyzed using qRT-PCR, immunofluorescence and in vitro functional assay for insulin and C-peptide release was assessed using insulin and C-peptide ELISA, respectively.. The cells grown under this altered culture conditions have exhibited a transition in the morphology from mesenchymal to epithelial with extensive piling up of cells. Article Title: Methods for making cells with an extra-embryonic endodermal precursor phenotype Article Snippet: DNAse treatment was performed using Turbo DNAse kit (Ambion 1907). cDNA synthesis was performed from 1 μg of RNA with Superscript III First-Strand synthesis system (Invitrogen 18080-051). .. Real time PCR was performed with SYBR Green Platinum SYBR green qPCR Supermix-UDG (Invitrogen 11733-046) in an Real-time Polymerase Chain Reaction:Article Title: Human pluripotent stem cell-derived hepatocytes support complete replication of hepatitis C virus. Article Snippet: Background & Aims: Worldwide, about 180 million people are Conclusions: Hepatocytes derived from hESC support the comchronically infected with the hepatitis C virus (HCV).. Current in vitro culture systems for HCV depend chiefly on human hepatoma cell lines.. Although primary human hepatocytes support HCV infection in vitro, and immunodeficient mice repopulated with human hepatocytes support HCV infection in vivo, these models are limited because of shortage of human livers to isolate hepatocytes. Article Title: Hmga2 translocation induced in skin tumorigenesis Article Snippet: RNA was isolated using the RNeasy Micro-kit (Qiagen), and DNase treatment with the Turbo DNAse kit (Ambion). cDNA synthesis was performed from 1 μg of RNA with Superscript III First-Strand synthesis system (Invitrogen). .. Quantitative Real time PCR (qRT-PCR) was performed using the Platinum SYBR green qPCR Supermix-UDG (Invitrogen) and the Article Title: Human Embryonic and Rat Adult Stem Cells with Primitive Endoderm-Like Phenotype Can Be Fated to Definitive Endoderm, and Finally Hepatocyte-Like Cells Article Snippet: DNAse treatment was performed using Turbo DNAse kit (Ambion 1907). cDNA synthesis was performed from 1 μg of RNA with Superscript III First-Strand synthesis system (Invitrogen 18080-051). .. Real time PCR was performed with SYBR Green Platinum SYBR green qPCR Supermix-UDG (Invitrogen 11733-046) in an Article Title: Lack of myostatin reduces MyoD induced myogenic potential of primary muscle fibroblasts. Article Snippet: Abbreviations used: αSMA, alpha smooth muscle actin; bHLH, basic helix loop helix; CAR, Coxackie and adenovirus receptor; CDK, cyclin dependent kinase; DMEM, dulbecco’s modified eagle’s medium; PBS, phosphate buffered saline; EDTA, ethylenediaminetetraacetic acid; Mstn, Myostatin; Mstn, myostatin null; MPP1, muscle preplate1; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; TGF-β, transforming growth factor beta; WT, wild-type; Article Title: NKX2-1 Activation by SMAD2 Signaling After Definitive Endoderm Differentiation in Human Embryonic Stem Cell Article Snippet: RNA was isolated using the RNeasy Mini-kit/Micro-kit (Qiagen), and DNAse treatment with the Turbo DNAse kit (Ambion). cDNA synthesis was performed from 1 μg of RNA with Superscript III First-Strand synthesis system (Invitrogen). .. Real time PCR was performed using the Platinum SYBR green qPCR Supermix-UDG (Invitrogen) and the Article Title: Non insulin producing cell line, MIA PaCa-2 is rendered insulin producing in vitro via mesenchymal epithelial transition. Article Snippet: We used non-insulin producing pancreatic carcinoma cell line, MIA PaCa-2 and have modulated its culture conditions by using 1% matrigel as extracellular matrix, N2, B27 growth supplements and serum free conditions.. Expression of markers was analyzed using qRT-PCR, immunofluorescence and in vitro functional assay for insulin and C-peptide release was assessed using insulin and C-peptide ELISA, respectively.. The cells grown under this altered culture conditions have exhibited a transition in the morphology from mesenchymal to epithelial with extensive piling up of cells. Article Title: Methods for making cells with an extra-embryonic endodermal precursor phenotype Article Snippet: DNAse treatment was performed using Turbo DNAse kit (Ambion 1907). cDNA synthesis was performed from 1 μg of RNA with Superscript III First-Strand synthesis system (Invitrogen 18080-051). .. Real time PCR was performed with SYBR Green Platinum SYBR green qPCR Supermix-UDG (Invitrogen 11733-046) in an |